phylogenetic typing and molecular detection of virulence factors of avian pathogenic escherichia coli isolated from colibacillosis cases in japanese quail

Authors

hesam alizade

infectious and tropical disease research center, hormozgan health institute, hormozgan university of medical sciences, bandar abbas, iran reza ghanbarpour

department of pathobiology, faculty of veterinary medicine, shahid bahonar university, kerman, iran maziar jajarami

department of pathobiology, faculty of veterinary medicine, shahid bahonar university, kerman, iran asma askari

department of pathobiology, faculty of veterinary medicine, shahid bahonar university, kerman, iran

abstract

colibacillosis caused by avian pathogenic escherichia coli (apec) is an economic threat to the poultry industry throughout the world. some of the virulence genes may enhance the ability of e. coli isolates to grow in the tissues of broilers. the apec strains are assigned to a few distinct phylogenetic groups. the purpose of the present study was to detect the virulence genes and phylogenetic groups of e. coli isolates from colibacillosis cases in japanese quail in 2014 in kerman, iran. in the present study, one hundred and two e. coli isolates were obtained from dead japanese quails with colibacillosis. e. coli isolates were confirmed by standard biochemical and bacteriological methods. dna of e. coli isolates was extracted by boiling method. the confirmed e. coli isolates were investigated to detect the phylogenetic groups and virulence genes including sfa/focde, afaibc, papef by pcr methods. e. coli isolates were classified into a (62 isolates), b1 (24 isolates), b2 (12 isolates) and d (four isolates) phylogenetic groups. among examined isolates nine isolates (8.82%) were positive for pape-f, five isolates (4.90%) for afaib-c and two isolates (1.96%) for sfa/focd-e genes. based on our findings, e. coli isolates from colibacillosis of japanese quail could be assigned to various phylogenetic groups (mostly a and d), and they may contain the adhesion genes in a low prevalence.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Phylogenetic typing and molecular detection of virulence factors of avian pathogenic Escherichia coli isolated from colibacillosis cases in Japanese quail

Colibacillosis caused by avian pathogenic Escherichia coli (APEC) is an economic threat to the poultry industry throughout the world. Some of the virulence genes may enhance the ability of E. coli isolates to grow in the tissues of broilers. The APEC strains are assigned to a few distinct phylogenetic groups. The purpose of the present study was to detect the virulence genes a...

full text

Phylogenetic typing and molecular detection of virulence factors of avian pathogenic Escherichia coli isolated from colibacillosis cases in Japanese quail

Colibacillosis caused by avian pathogenic Escherichia coli (APEC) is an economic threat to the poultry industry throughout the world. Some of the virulence genes may enhance the ability of E. coli isolates to grow in the tissues of broilers. The APEC strains are assigned to a few distinct phylogenetic groups. The purpose of the present study was to detect the virulence genes and phylogenetic gr...

full text

Distribution of virulence associated genes in isolated Escherichia coli from avian colibacillosis

BACKGROUND: Colibacillosis is one of the most prevalentdiseases in the world that causes multimillion-dollar annuallosses. OBJECTIVES: In order to evaluate molecular epidemiologyof some virulence associated factors in Escherichia coli,isolated from poultry, the presence of iut A, iss, hly F, omp T, iroN, afa, sfa (S)and pap G (II) were investigated by multiplex PCRassay. METHODS: Two hundred th...

full text

Antibiotic Resistance Pattern of Different Escherichia coli Phylogenetic Groups Isolated from Human Urinary Tract Infection and Avian Colibacillosis

Background: The emergence and propagation of different phylogenetic groups of antimicrobial-resistant E. coli have become a worldwide health concern in human and veterinary medicine. Therefore, the evaluation of the phylogenetic distribution of antibiotic-resistant E. coli is important for therapeutic and economic purposes. The aims of this study were to determine phylogenetic groups and patter...

full text

Detection of eaeA, hlyA, stx1 and stx2 genes in pathogenic Escherichia coli isolated from broilers affected with colibacillosis

Background: Foodborne outbreaks associated with shiga toxin-producing Escherichia coli (STEC) have been well documented worldwide. STECs are major causative agents of gastroenteritis in humans that may be complicated by hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS), and thrombotic thrombocytopenia purpura (TTP). OBJECTIVES: The aim of this study was to investigate the presence of vi...

full text

Extra intestinal pathogenic Escherichia coli from human and avian origin: Detection of the most common virulence-encoding genes

Pathogenic Escherichia coli strains cause a wide range of extra intestinal infections including urinary tract infection in humans and colibacillosis in poultry. They are classified into uropathogenic E. coli (UPEC) and avian pathogenic E. coli (APEC) with genetic similarities and variations. Their pathogenicity is related to the virulence-encoding genes like sfa</...

full text

My Resources

Save resource for easier access later


Journal title:
veterinary research forum

جلد ۸، شماره ۱، صفحات ۵۵-۵۸

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023